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ViewSizerTM 3000

BrochureDownload ViewSizerTM 3000 Brochure
The ViewSizerTM 3000 is an instrument that visualises scattering from individual nanoparticles in suspension. This data is used to determine particle movement and infer particle size using the Stokes Einstein Relationship. Then, by using the known illuminated and imaged sample volume, particle number concentration is readily determined. Thus, from a single measurement, two critical pieces of information are determined: Particle size distribution and particle concentration.

By incorporating three variable light sources (blue, green and red), the The ViewSizer 3000 is able to select the optimum conditions for any sample analysis, overcoming the limitations of NTA when analysing polydisperse sample and enabling a much larger range of particle sizes to be visualized.

By introducing the sample in an easy-to-use (and clean) cuvette, the ViewSizer 3000 is able to repeatedly "analyze and stir", giving a much more reproducible result. And because the sample is viewed in a vertical orientation, it is perfect for visualising processes such as protein aggregation or crystal dissolution. ?

Whether you are using NTA for the analysis of exosomes or other microvesicles or for complex nanoparticle kinetic processes, the ViewSizer 3000 is a breakthrough technology, bringing better analysis to your laboratory.
ViewSizer Schematic

Key Features

  • High resolution particle size distribution and visualisation
  • Wide range of particle sizes in the same sample
  • Multiple light sources (blue, green and red)
  • Perfect for monitoring nanoparticle kinetics

Instrument Specifications

Measurement Range 10 nm to 15 µm
Typical Sample Volume 350 µL to 2.5 mL
Typical Sample Concentration (Sample dependent) 10^5 - 10^9 particles/mL
Sample Temperature Range (Controlled) 10° C to 50° C +/- 0.1° C
Dimensions 55 cm W x 66 cm D x 35 cm H
Weight 27 kg
Operational Environment 15° C to 30° C with < 85% RH

Particle Tracking Standards

NTA Standards Well accepted data can't be obtained without international standards. Dr Koltsov is leading the development and revision of the NTA/PTA ISO standards (ISO 19430) since 2013. The new version of the document allows for particle sizing, particle size distribution and particle counting that leads to particle number concnetration. 3-Laser systems, as well as the gravitational fall sizing are documented. It should also be noted that E2834-12(2022) is also used. Why is this importnat? This means that a certified lab operating under ISO 17025 can offer certified measurement reports.
HTML Link ISO 19430:2016 Particle size analysis - Particle tracking analysis (PTA) method
HTML Link ASTM E2834-12(2018) Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis (NTA)

Applications Notes

app Enhanced Characterization of Fluorescently Labeled Extracellular Vesicles using ViewSizer 3000. Exosomes are small extracellular vesicles, 30-150 nm in diameter, which have been determined to play a crucial role in extracellular signaling.Exosomes are small extracellular vesicles, 30-150 nm in diameter, which have been determined to play a crucial role in extracellular signaling.
app Particle Analysis in Vaccine Manufacturing and Development
app Achieving Infectious Titer with Multi-laser Nanoparticle Tracking Analysis (NTA)
app Optimization of an Emulsion Polymerization Process and Product Through Nanoparticle Concentration Analysis
app Predicting Whiskey Shelf Stability with Particle Size Distribution
app Optimization of High-Performance Nanostructured Powder Metallurgy Materials
app Nanobubble Size and Concentration Analysis with Multi-laser Nanoparticle Tracking Analysis (NTA)
app Accurate Protein Aggregation Analysis
app Size Distribution and Concentration of Exosomes
app Characterization of Sub-Visible Particles in Protein Therapeutic Formulations
app Nanoparticle Analysis
app Metal Powder Size Measurement
app Particle Analysis in Polymers & Plastics
app Quantifying Trace Amount of Nanoparticles in Drinking Water
app Viruses and Virus-Like Particles

Videos and Tutorials

map Not all nanoparticle questions are the same. There are a number of options for characterizing submicron particles and the right choice requires considering more than just a single specification such as minimum particle size. In this webinar, Dr. Jeff Bodycomb introduces, compares, and contrasts the three main techniques for nanoparticle analysis: Dynamic Light Scattering (DLS), Laser Diffraction, and Nanoparticle Tracking Analysis (NTA), including the latest advances. He includes applications and information to help you think about the best way to get the answers you need.
map Multi-laser Nanoparticle Tracking Analysis (NTA) is an advanced patented technology featuring three simultaneously operating lasers with independently adjustable power. This enables broad size distributions of, for example, biologics particle mixtures to be accurately analyzed for size and concentration. This webinar offers a broad overview on how multi-laser NTA technique fits into the gene therapy space and where we and our collaborators see the applications going in the near future.
map In this webinar, Dr. Pushpendra Singh, of Walter Reed Army Institute of Research, discusses the significance of liposome size characteristics in medicine, including the challenges present in particle size measurement for heterogeneous size containing formulation (polydisperse). He will also discuss his recently published results on polydisperse bead and liposome formulations using DLS, conventional Nanoparticle Tracking Analysis (NTA), laser diffraction and a novel multispectral NTA measurement techniques.
map Accurate concentration for virus and virus-like particles can be determined by multi-laser nanoparticle tracking analysis due to the fact they are nanoparticles. Other biologically relevant materials will have sizes that are close to those of viruses, whether they are protein aggregates that provoke an unwanted immune response or exosomes with a similar size and do not provoke an immune response

Featured Publications for the ViewSizer 3000

These are the select publications relevant to or using ViewSizer 3000. This list will be updated periodically.
HTML Link Biological characterization using protein crystal measurements
by William Wittbold and Kuba Tatarkiewicz (2017)
HTML Link A lipase-independent pathway of lipid release and immune modulation by adipocytes
SCIENCE, 1 Mar 2019, Vol 363, Issue 6430, pp. 989-993 DOI: 10.1126/science.aaw2586
HTML Link Application of a novel new multispectral nanoparticle tracking technique
HTML Link Biophysical characterization of polydisperse liposomal adjuvant formulations
HTML Link Characterisation of particles in solution - a perspective on light scattering and comparative technologies
HTML Link Cyclodextrin Reduces Intravenous Toxicity of a Model Compound
HTML Link Development and anti-Candida evaluation of the vaginal delivery system of ... nanosuspension-loaded thermogel
HTML Link Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol
HTML Link Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity
HTML Link Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis
HTML Link Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye
HTML Link Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology
HTML Link Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control
HTML Link Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder
HTML Link Synthesis and Characterization of EGFR-Targeted Immunoporphysomes
HTML Link Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions
HTML Link Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles | Protocol (
HTML Link Isolation and characterization of EV in Saliva of Children with Asthma
HTML Link Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential
HTML Link Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo
HTML Link Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
HTML Link Purification of Cas9 - RNA Complexes by Ultrafiltration
HTML Link Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications
HTML Link Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration
HTML Link Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo

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