ViewSizerTM 3000
Download ViewSizerTM 3000 Brochure
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The ViewSizerTM 3000 is an instrument that visualises scattering from individual nanoparticles in suspension. This data is used to determine particle movement and infer particle size using the Stokes Einstein Relationship. Then, by using the known illuminated and imaged sample volume, particle number concentration is readily determined. Thus, from a single measurement, two critical pieces of information are determined: Particle size distribution and particle concentration.
By incorporating three variable light sources (blue, green and red), the The ViewSizer 3000 is able to select the optimum conditions for any sample analysis, overcoming the limitations of NTA when analysing polydisperse sample and enabling a much larger range of particle sizes to be visualized.
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By introducing the sample in an easy-to-use (and clean) cuvette, the ViewSizer 3000 is able to repeatedly "analyze and stir", giving a much more reproducible result. And because the sample is viewed in a vertical orientation, it is perfect for visualising processes such as protein aggregation or crystal dissolution.
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Whether you are using NTA for the analysis of exosomes or other microvesicles or for complex nanoparticle kinetic processes, the ViewSizer 3000 is a breakthrough technology, bringing better analysis to your laboratory.
Key Features
- High resolution particle size distribution and visualisation
- Wide range of particle sizes in the same sample
- Multiple light sources (blue, green and red)
- Perfect for monitoring nanoparticle kinetics
Instrument Specifications
Measurement Range
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10 nm to 15 µm
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Typical Sample Volume
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350 µL to 2.5 mL
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Typical Sample Concentration
(Sample dependent)
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10^5 - 10^9 particles/mL
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Sample Temperature Range
(Controlled)
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10° C to 50° C +/- 0.1° C
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Dimensions
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55 cm W x 66 cm D x 35 cm H
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Weight
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27 kg
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Operational Environment
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15° C to 30° C with < 85% RH
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Particle Tracking Standards

Well accepted data can't be obtained without international standards. Dr Koltsov is leading the development and revision of the NTA/PTA ISO standards (
ISO 19430) since 2013. The new version of the document allows for particle sizing, particle size distribution and particle counting that leads to particle number concnetration. 3-Laser systems, as well as the gravitational fall sizing are documented. It should also be noted that
E2834-12(2022) is also used. Why is this importnat? This means that a certified lab operating under ISO 17025 can offer certified measurement reports.
Applications Notes
Videos and Tutorials
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Not all nanoparticle questions are the same. There are a number of options for characterizing submicron particles and the right choice requires considering more than just a single specification such as minimum particle size. In this webinar, Dr. Jeff Bodycomb introduces, compares, and contrasts the three main techniques for nanoparticle analysis: Dynamic Light Scattering (DLS), Laser Diffraction, and Nanoparticle Tracking Analysis (NTA), including the latest advances. He includes applications and information to help you think about the best way to get the answers you need.
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Multi-laser Nanoparticle Tracking Analysis (NTA) is an advanced patented technology featuring three simultaneously operating lasers with independently adjustable power. This enables broad size distributions of, for example, biologics particle mixtures to be accurately analyzed for size and concentration. This webinar offers a broad overview on how multi-laser NTA technique fits into the gene therapy space and where we and our collaborators see the applications going in the near future.
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In this webinar, Dr. Pushpendra Singh, of Walter Reed Army Institute of Research, discusses the significance of liposome size characteristics in medicine, including the challenges present in particle size measurement for heterogeneous size containing formulation (polydisperse). He will also discuss his recently published results on polydisperse bead and liposome formulations using DLS, conventional Nanoparticle Tracking Analysis (NTA), laser diffraction and a novel multispectral NTA measurement techniques.
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Accurate concentration for virus and virus-like particles can be determined by multi-laser nanoparticle tracking analysis due to the fact they are nanoparticles. Other biologically relevant materials will have sizes that are close to those of viruses, whether they are protein aggregates that provoke an unwanted immune response or exosomes with a similar size and do not provoke an immune response
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Featured Publications for the ViewSizer 3000
These are the select publications relevant to or using ViewSizer 3000. This list will be updated periodically.
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Biological characterization using protein crystal measurements
by William Wittbold and Kuba Tatarkiewicz
(2017)
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A lipase-independent pathway of lipid release and immune modulation by adipocytes
by
STEPHEN E. FLAHERTY, AMBAR GRIJALVA, XIAOYUAN XU, ELEANORE ABLES, ALIREZA NOMANI, AND ANTHONY W. FERRANTE JR. SCIENCE, 1 Mar 2019, Vol 363, Issue 6430, pp. 989-993
DOI: 10.1126/science.aaw2586
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Application of a novel new multispectral nanoparticle tracking technique
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Biophysical characterization of polydisperse liposomal adjuvant formulations
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Characterisation of particles in solution - a perspective on light scattering and comparative technologies
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Cyclodextrin Reduces Intravenous Toxicity of a Model Compound
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Development and anti-Candida evaluation of the vaginal delivery system of ... nanosuspension-loaded thermogel
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Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol
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Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity
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Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis
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Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells https://doi.org/10.1038/s41598-020-67039-w
Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye
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Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology
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Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control
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Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder
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Synthesis and Characterization of EGFR-Targeted Immunoporphysomes
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Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions
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Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles | Protocol (jove.com)
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Isolation and characterization of EV in Saliva of Children with Asthma
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Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential
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Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo
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Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
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Purification of Cas9 - RNA Complexes by Ultrafiltration
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Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications
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Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration
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Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo
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Contact your Distributor
Email:
denis@brec-solutions.com